5-Bromo-4-Chloro-3-indolyl a-D-galactopyranoside
{X-alpha-Gal}
X-a-Gal is a chromogenic substrate which can be used to demonstrate a-galactosidase activity. Glycosynth offers this product in high purity (>98%) at a competitive price. |
Code |
Product |
Quantity |
Price Sterling |
Price USD |
Price Euro |
70039 |
X-a-Gal |
250mg |
£110 |
$215 |
182 |
| |
To place an order, please contact us by phone, fax or e-mail. Alternatively, click here to use our on-line order form. To view our other high purity enzyme substrates, please visit our home page | |
|
X-a-Gal and the Yeast Two-Hybrid Screen
The yeast two-hybrid screen is a genetic assay to detect protein-protein interactions in vivo. This method, developed by Prof. Stan Fields and coworkers (1) can be used to investigate the interaction of two known proteins (e.g. defining domains or amino acids critical for interactions). Another important application is the identification of
|
How to make X-a-Gal Indicator Plates To make X-a-Gal indicator plates, X-a-Gal can be either spread on top of the medium or included in the medium prior to pouring the plates. |
References |
1.) |
A colony-colour method which differentiates a-galactosidase positive strains of yeast. R.S. Tubb and P.L. Liljestrom, J. Inst. Brew. 92, 588, (1986) |
2.) |
Histochemical detection of a-D-galactosidase with 5-Br-4-Cl-3-indoxyl a-D-galactoside R. Gossrau and Z. Lojda, Acta histochem., 85, 213 (1989) |
3.) |
X-a-gal-based medium for simultaneous enumeration of bifidobacteria and lactic acid bacteria in milk. P. Chevalier, D. Roy and and L. Savoie, J.Microbiol. Meth., 13, 75 (1991) |
4.) |
American Society of Brewing Chemists. Report of Subcommittee on X-a-gal medium. J. Amer. Soc. Brew. Chem., 51, 185, (1993) |