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Chromogenic DHN and Brown Substrates

Our new range of chromogenic substrates are based on the core molecule 2,3-dihydroxynaphthalene (DHN, product code 80XXX). DHN-substrates are colourless; however, when cleaved in the presence of an iron salt they produce an intense, highly-visible, purple colour. The substrates are suitable for use in liquid or tube media under both aerobic and anaerobic conditions. They offer a number of advantage over other commonly used substrates, one major advantage being that the intense purple colour is able to mask the yellow produced by ONPG cleavage, making DHN-substrates especially suitable for dual chromogenic systems. 

The novel range of 6,7-Dibromo-2,3-Dihydroxynaphthalene (Brown, product code 56XXX) substrates for use in agar plates for the detection of Gram-negative organisms under both aerobic and anaerobic conditions. They offer easy detection of any enzyme-positive reaction by eye, when used with an iron salt in the growth medium, giving discreate red-brown coloured colonies on the plates.

DHN and Brown substrates are protected by European Patents EP3066107, EP3066209 and US Patents US9879303US9938562, US10443084 if appropriate, as indicated under each product.

Code Item
80045 2,3-Dihydroxynaphthalene beta-D-galactopyranoside
80059 2,3-Dihydroxynaphthalene beta-D-glucopyranoside
80065 2,3-Dihydroxynaphthalene beta-D-glucuronide cyclohexylammonium salt
80180 2,3-Dihydroxynaphthalene beta-D-ribofuranoside
56045 6,7-Dibromo-2,3-Dihydroxynaphthalene beta-D-galactopranoside
56059 6,7-Dibromo-2,3-Dihydroxynaphthalene beta-D-glucopranoside
56065 6,7-Dibromo-2,3-Dihydroxynaphthalene beta-D-glucuronide cyclohexylammonium salt
56066 6,7-Dibromo-2,3-Dihydroxynaphthalene beta-D-glucuronide sodium salt
56180 6,7-Dibromo-2,3-Dihydroxynaphthalene beta-D-ribofuranoside

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Glycosynth Limited
14 Craven Court, Winwick Quay, Warrington, Cheshire. WA2 8QU England
Tel : 01925 575075       Fax : 01925 575121