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Chromogenic Glucuronide Substrates

These novel chromogenic substrates for the detection of beta-D-glucuronidase activity, 2,3-Dihydroxynaphthalene β-D-glucuronide (DHN β-D-glucuronide, product code: 80065 CHA salt and 80066 sodium salt) and 2-Hydroxyphenyl β-D-glucuronide (Catechol β-D-glucuronide, product code 14065 CHA salt and 14066 sodium salt), give highly-visible purple and black colours (respectively) when cleaved in the presence of an iron salt, under both aerobic and anaerobic conditions. They offer a number of advantage over other commonly used substrates, one major advantage being that these dark colours show a remarkable contrast between positive and negative bacterial cultures and are able to mask the yellow produced by ONPG cleavage, making them particularly suitable for dual chromogenic systems.

Catechol and DHN substrates are protected by European Patents EP3066107, EP3066209 and US Patents US9879303, US9938562 and US10443084 if appropriate, as indicated under each product.

Code Item
80065 2,3-Dihydroxynaphthalene beta-D-glucuronide cyclohexylammonium salt
14065 2-Hydroxyphenyl beta-D-glucuronide cyclohexylammonium salt
14066 2-Hydroxyphenyl beta-D-glucuronide sodium salt

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